Study of bi-enzyme immobilization onto layered double hydroxides nanomaterials for histamine biosensor application

Abstract : In this work, we present the development of a hybrid biomembrane based on the immobilization of diamine oxidase (DAO) into LDH thin films for histamine detection. The LDHs preselected as host matrixes are: hydrotalcites (Mg2Al(CO3)0.5(OH)6), Iowaite (Mg4Fe(OH)10Cl) and hydrocalumite (Ca2Al(OH)6Cl). The immobilized probes were characterized by atomic force microscopy (AFM) and attenuated total reflection infrared spectroscopy (IR-ATR mode). The analysis of these results shows that the immobilization of DAO occurs with all type of selected LDH and is stable after a 7 day-immersion in phosphate buffer solution. The LDH incorporating magnesium or calcium divalent cations present high-quality surface topology for DAO immobilization and the ability to keep the enzyme in a well conformation for biogenic amines catabolism and histamine detection.
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Submitted on : Friday, June 13, 2014 - 3:26:07 PM
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Zouhair M. Baccar, S. Hidouri, Abdelhamid Errachid, O. Ruiz Sanchez. Study of bi-enzyme immobilization onto layered double hydroxides nanomaterials for histamine biosensor application. Journal of Nanoscience and Nanotechnology, American Scientific Publishers, 2011, 11 (10), pp.8798-8803. ⟨10.1166/jnn.2011.3461⟩. ⟨hal-01006037⟩

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