Lactococcus lactis as a live vector: Heterologous protein production and DNA delivery systems

Daniela Santos D. S. Pontes (1) , Marcela Santiago M. S. Pacheco de Azeyedo (1) , Jean-Marc J.-M. Chatel (2) , Philippe P. Langella (2) , Vasco V. Azeyedo (1) , Anderson A. Miyoshi (1)

1 Laboratory of Cellular and Molecular Genetics
2 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé

Lactic acid bacteria (LAB), widely used in the food industry, are present in the intestine of most animals, including humans. The potential use of these bacteria as mucosal delivery vehicles for vaccinal, medical or technological use has been extensively investigated. Lactococcus Iactis, a Lspecies, is a potential candidate for the production of biologically useful proteins and for plasmid DNA delivery to eukaryotic cells. Several delivery systems have been developed to target heterologous proteins to a specific cell location (i.e., cytoplasm, cell wall or extracellular medium) and more recently to efficiently transfer DNA to eukaryotic cells. A promising application of L lactis is its use for the development of live mucosal vaccines. Here, we have reviewed the expression of heterologous protein and the various delivery systems developed for L. lactis, as well as its use as an oral vaccine carrier. (C) 2011 Elsevier Inc. All rights reserved.

Mots clés: Lactic acid bacteria; Lactococcus lactis; Heterologous expression; Delivery systems; Vaccines; CONTROLLED GENE-EXPRESSION; TOXIN FRAGMENT-C; RECOMBINANT LACTOBACILLUS-PLANTARUM; STAPHYLOCOCCUS-HYICUS LIPASE; MAMMALIAN EPITHELIAL-CELLS; MUCOSAL IMMUNE-RESPONSES; GRAM-POSITIVE BACTERIA; ACID BACTERIA; TETANUS TOXIN; STREPTOCOCCUS-PYOGENES

Domaines: Sciences agricoles

Daniela Santos D. S. Pontes, Marcela Santiago M. S. Pacheco de Azeyedo, Jean-Marc J.-M. Chatel, Philippe P. Langella, Vasco V. Azeyedo, et al.. Lactococcus lactis as a live vector: Heterologous protein production and DNA delivery systems. Protein Expression and Purification, 2011, 79 (2), pp.165 - 175. ⟨10.1016/j.pep.2011.06.005⟩. ⟨hal-01000980⟩