Expression and localization of DLX3, PPARG and SP1 in bovine trophoblast during binucleated cell differentiation

Severine S. Degrelle (1) , P. P. Murthi (2, 3) , D. D. Evain-Brion (4, 5, 6) , T. T. Fournier (4, 5, 6) , Isabelle Hue (1)

1 BDR - Biologie du développement et reproduction
2 Department of Perinatal Medicine, Pregnancy Research Centre
3 Department of Obstetrics and Gynaecology [Melbourne]
4 UMR_S 767 - La grossesse normale et pathologique: développement et fonctions du placenta et de l'utérus
5 UPD5 - Université Paris Descartes - Paris 5
6 PremUp Foundation

The differentiation of the trophoblast is marked in ruminants by the formation of binucleated cells (BNC). They appear from pre-implantation onwards but the molecular mechanisms underlying their differentiation remain largely unexplored. Taking advantage of our recent data, we analyzed the expression pattern of DLX3 and PPARG that are known regulators of early placenta formation and extended our analysis to one of their potential regulators, SP1. Our study is the first to demonstrate the co-expression of DLX3, PPARG and SP1 in bovine BNC nuclei. This suggests a possible role of these transcription factors through BNC specific genes at the time of pre-placental differentiation.

Mots clés: bovine; trophoblaste; differentiation

Domaines: Biologie de la reproduction; Biologie du développement

Severine S. Degrelle, P. P. Murthi, D. D. Evain-Brion, T. T. Fournier, Isabelle Hue. Expression and localization of DLX3, PPARG and SP1 in bovine trophoblast during binucleated cell differentiation. Placenta, 2011, 32 (11), pp.917-920. ⟨10.1016/j.placenta.2011.08.014⟩. ⟨hal-01000203⟩