Development of on-line comprehensive two-dimensional liquid chromatography method for the separation of biomass compounds

Abstract : Comprehensive on-line two-dimensional liquid chromatography (on-line LC × LC) was used for the characterization of bio-oils obtained by fast pyrolysis of lignocellulosic biomass. The resulting bio-oil contains a large number of oxygenated chemical families and must therefore be upgraded before being used as drop-in transportation biofuels. The good knowledge of its complex composition is essential for optimizing the mandatory bio-oil upgrading process to biofuels, thereby requiring powerful separation techniques designed to be hyphenated to mass spectrometry detection (LC × LC-MS). In this study, reversed phase conditions were optimized in both dimensions for the RPLC × RPLC separation of the aqueous fraction of bio-oils. The first step of method development consisted in searching for a suitable set of RP-conditions via the screening of a large number of RP-systems (made up of different stationary phases and/or mobile phases and/or temperature). The practical peak capacity and the degree of orthogonality were calculated for a sample of 38 representative compounds, both descriptors having been considered as selection criterion. Two different couplings were chosen and evaluated for the RPLC × RPLC separation of the 38 representative compounds. The best of both, in terms of real practical peak capacity, was further successfully applied to the separation of the aqueous phase of a partially dehydroxygenated bio-oil.
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https://hal.archives-ouvertes.fr/hal-00991030
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Submitted on : Wednesday, May 14, 2014 - 3:45:30 PM
Last modification on : Thursday, February 7, 2019 - 4:49:24 PM

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Agnès Le Masle, David Angot, Cécile Gouin, Amélie d'Attoma, Alain Quignard, et al.. Development of on-line comprehensive two-dimensional liquid chromatography method for the separation of biomass compounds. Journal of Chromatography A, Elsevier, 2014, 1340 (2), pp.90-98. ⟨10.1016/j.chroma.2014.03.020⟩. ⟨hal-00991030⟩

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