Influence of composition of zeolite/enzyme nanobiocomposites on analytical characteristics of urea biosensor based on ion-selective field-effect transistors

Abstract : Zeolite/enzyme nanobiocomposites of different compositions were tested in this work for the improvement of biosensor analytical characteristics. The bioselective element based on urease immobilized by cross-linking with glutaraldehyde was used as a model. The working characteristics of biosensors based on various zeolite/enzyme nanocomposites were examined and compared with those of urease-based biosensors. An optimal concentration of zeolytes beta (BEA) in bioselective elements is determined to be 1.5%. It ensures as wide linear range of measurement without remarkable loss in biosensor sensitivity to urea. The BEA zeolite-based biosensors were shown to have better working parameters in comparison with those based on zeolites A (LTA). A decrease in biosensor sensitivity to heavy metal ions was demonstrated for all zeolites used, which testifies to probable increase in stability of urea measurement in real environmental samples.
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Submitted on : Sunday, May 4, 2014 - 7:35:28 PM
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O. O. Soldatkin, E. Soy, Abdelhamid Errachid, Nicole Jaffrezic-Renault, B. Akata, et al.. Influence of composition of zeolite/enzyme nanobiocomposites on analytical characteristics of urea biosensor based on ion-selective field-effect transistors. 7th Maghreb-Europe Meeting on Materials and Their Applications for Devices and Sensors (MADICA 2010), Oct 2010, Tabarka, Tunisia. Sensor letters, 9 (6), pp.2320-2326, 2011, ⟨10.1166/sl.2011.1809⟩. ⟨hal-00986753⟩

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