Gold electrode functionalized with catalase for impedimetric detection of nitrite

Abstract : The principle of the developed biosensor includes the following: Catalase catalyzed the breakdown of H2O2 into H2O and O2. Nitrite was selected as an inhibitor of catalase. Faradaic Electrochemical Impedance Spectroscopy (EIS) has been used in our work as a tool for the characterization of a new nitrite biosensor for environmental applications based on the immobilization of catalase on gold electrode. Under optimal conditions, i.e., buffer capacity corresponding to 3 mM phosphate buffer, the catalase enzyme biomembrane shows a high sensitivity to nitrite inhibition. In both cases, the responses of these biosensors based on nitrite additions are good with a detection limit around 8 × 10−11 M.
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Submitted on : Sunday, May 4, 2014 - 7:16:54 PM
Last modification on : Friday, October 19, 2018 - 11:20:02 AM

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A. Zazoua, C. Dernane, I. Kazane, M. Belghobsi, Abdelhamid Errachid, et al.. Gold electrode functionalized with catalase for impedimetric detection of nitrite. 7th Maghreb-Europe Meeting on Materials and Their Applications for Devices and Sensors (MADICA 2010), Oct 2010, Tabarka, Tunisia. Sensor letters, 9 (6), pp.2283-2286, 2011, ⟨10.1166/sl.2011.1815⟩. ⟨hal-00986750⟩

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