Conductometric biosensors based on layer-by-layer coated paramagnetic nanoparticles for urea detection

W. Nouira (1) , Abderrazak Maaref (2) , M. Siadat , Abdelhamid Errachid (1) , Nicole Jaffrezic-Renault (1)

1 SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014)
2 LIMA - Laboratoire des Interfaces et Matériaux Avancés [Monastir]

An interdigitated conductometric biosensor for the detection of urea was obtained by functionalization of magnetic nanoparticles with polyelectrolyte:Poly(allylamine hydrochloride) (PAH), for urease immobilization, using the method of layer-by-layer deposition (LbL). The maximum of response obtained with nanoparticles is 39 μS compared to 20 μS for urease directly cross-linked on the sensor. The dynamic range is very much enlarged: from 0.1 mM to 10 mM with urease-PAH coated nanoparticles and 1 mM to 5 mM for directly cross-linked urease on the sensor. The biosensor was stable over a period of 30 days.

Domaines: Chimie analytique

W. Nouira, Abderrazak Maaref, M. Siadat, Abdelhamid Errachid, Nicole Jaffrezic-Renault. Conductometric biosensors based on layer-by-layer coated paramagnetic nanoparticles for urea detection. 7th Maghreb-Europe Meeting on Materials and Their Applications for Devices and Sensors (MADICA 2010), Oct 2010, Tabarka, Tunisia. Sensor letters, 9 (6), pp.2272-2274, 2011, ⟨10.1166/sl.2011.1805⟩. ⟨hal-00986748⟩