Conductometric biosensors based on layer-by-layer coated paramagnetic nanoparticles for urea detection

W. Nouira 1 Abderrazak Maaref 2 M. Siadat Abdelhamid Errachid 1 Nicole Jaffrezic-Renault 1
1 SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes
ISA - Institut des Sciences Analytiques
2 LIMA - Laboratoire des Interfaces et Matériaux Avancés
Abstract : An interdigitated conductometric biosensor for the detection of urea was obtained by functionalization of magnetic nanoparticles with polyelectrolyte:Poly(allylamine hydrochloride) (PAH), for urease immobilization, using the method of layer-by-layer deposition (LbL). The maximum of response obtained with nanoparticles is 39 μS compared to 20 μS for urease directly cross-linked on the sensor. The dynamic range is very much enlarged: from 0.1 mM to 10 mM with urease-PAH coated nanoparticles and 1 mM to 5 mM for directly cross-linked urease on the sensor. The biosensor was stable over a period of 30 days.
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Submitted on : Sunday, May 4, 2014 - 7:10:10 PM
Last modification on : Wednesday, September 12, 2018 - 10:00:05 AM

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ISA | ENS-LYON | INC-CNRS | UNIV-LYON1

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W. Nouira, Abderrazak Maaref, M. Siadat, Abdelhamid Errachid, Nicole Jaffrezic-Renault. Conductometric biosensors based on layer-by-layer coated paramagnetic nanoparticles for urea detection. 7th Maghreb-Europe Meeting on Materials and Their Applications for Devices and Sensors (MADICA 2010), Oct 2010, Tabarka, Tunisia. Sensor letters, 9 (6), pp.2272-2274, 2011, ⟨10.1166/sl.2011.1805⟩. ⟨hal-00986748⟩

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