Fast Proton Exchange in Histidine: Measurement of Rate Constants through Indirect Detection by NMR Spectroscopy.

Akansha Ashvani Sehgal , Luminita Duma (1) , Geoffrey Bodenhausen (1) , Philippe Pelupessy (1)

1 UPMC - Université Pierre et Marie Curie - Paris 6

: Owing to its imidazole side chain, histidine participates in various processes such as enzyme catalysis, pH regulation, metal binding, and phosphorylation. The determination of exchange rates of labile protons for such a system is important for understanding its functions. However, these rates are too fast to be measured directly in an aqueous solution by using NMR spectroscopy. We have obtained the exchange rates of the NH3 (+) amino protons and the labile NH(ε2) and NH(δ1) protons of the imidazole ring by indirect detection through nitrogen-15 as a function of temperature (272 K

Domaines: Chimie organique

Akansha Ashvani Sehgal, Luminita Duma, Geoffrey Bodenhausen, Philippe Pelupessy. Fast Proton Exchange in Histidine: Measurement of Rate Constants through Indirect Detection by NMR Spectroscopy.. Chemistry - A European Journal, 2014, 20 (21), pp.6332-6338. ⟨10.1002/chem.201304992⟩. ⟨hal-00978290⟩