Caspase-11 Controls Interleukin-1β Release through Degradation of TRPC1.

Bénédicte F Py , Mingzhi Jin , Bimal N Desai , Anirudh Penumaka , Hong Zhu , Maike Kober , Alexander Dietrich , Marta M Lipinski , Thomas Henry (1) , David E Clapham , Junying Yuan (2)

1 I2V - Immunité infection vaccination
2 Department of cell biology

Caspase-11 is a highly inducible caspase that controls both inflammatory responses and cell death. Caspase-11 controls interleukin 1β (IL-1β) secretion by potentiating caspase-1 activation and induces caspase-1-independent pyroptosis downstream of noncanonical NLRP3 inflammasome activators such as lipopolysaccharide (LPS) and Gram-negative bacteria. However, we still know very little about the downstream mechanism of caspase-11 in regulating inflammation because the known substrates of caspase-11 are only other caspases. Here, we identify the cationic channel subunit transient receptor potential channel 1 (TRPC1) as a substrate of caspase-11. TRPC1 deficiency increases the secretion of IL-1β without modulating caspase-1 cleavage or cell death in cultured macrophages. Consistently, trpc1(-/-) mice show higher IL-1β secretion in the sepsis model of intraperitoneal LPS injection. Altogether, our data suggest that caspase-11 modulates the cationic channel composition of the cell and thus regulates the unconventional secretion pathway in a manner independent of caspase-1.

Domaines: Sciences du Vivant [q-bio]

Bénédicte F Py, Mingzhi Jin, Bimal N Desai, Anirudh Penumaka, Hong Zhu, et al.. Caspase-11 Controls Interleukin-1β Release through Degradation of TRPC1.. Cell Reports, 2014, 6 (6), pp.1122-8. ⟨10.1016/j.celrep.2014.02.015⟩. ⟨hal-00972069⟩