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Laser Doppler holographic microscopy in transmission: application to fish embryo imaging

Abstract : We have extended Laser Doppler holographic microscopy to transmission geometry. The technique is validated with living fish embryos imaged by a modified upright bio-microcope. By varying the frequency of the holographic reference beam, and the combination of frames used to calculate the hologram, multimodal imaging has been performed. Doppler images of the blood vessels for different Doppler shifts, images where the flow direction is coded in RGB colors or movies showing blood cells individual motion have been obtained as well. The ability to select the Fourier space zone that is used to calculate the signal, makes the method quantitative.
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https://hal.archives-ouvertes.fr/hal-00970195
Contributor : Michel Gross <>
Submitted on : Wednesday, April 30, 2014 - 1:52:30 PM
Last modification on : Wednesday, October 14, 2020 - 4:19:05 AM
Long-term archiving on: : Wednesday, July 30, 2014 - 10:45:11 AM

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Nicolas Verrier, Daniel Alexandre, Michel Gross. Laser Doppler holographic microscopy in transmission: application to fish embryo imaging. Optics Express, Optical Society of America - OSA Publishing, 2014, 22, pp.9368. ⟨10.1364/OE.22.009368⟩. ⟨hal-00970195⟩

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