Contribution of amoebic coculture to recovery of Legionella isolates from respiratory samples: prospective analysis over a period of 32 months.

G. Descours , A. Suet , C. Ginevra , C. Campese , S. Slimani , F. Ader (1, 2) , D. Che , G. Lina (3) , S. Jarraud

1 Hosp Civils Lyon, Serv Malad Infect, Lyon, France
2 LCME - Laboratoire de chimie et microbiologie de l'eau [Poitiers]
3 I2V - Immunité infection vaccination

We evaluated the contribution of amoebic coculture to the recovery of Legionella spp. from 379 respiratory samples. The sensitivity of axenic culture was 42.1%. The combination of axenic culture with amoebic coculture increased the Legionella isolation rate to 47.1%. Amoebic coculture was particularly efficient in isolating Legionella spp. from respiratory samples contaminated with oropharyngeal flora.

Domaines: Sciences du Vivant [q-bio]

G. Descours, A. Suet, C. Ginevra, C. Campese, S. Slimani, et al.. Contribution of amoebic coculture to recovery of Legionella isolates from respiratory samples: prospective analysis over a period of 32 months.. Journal of Clinical Microbiology, 2012, 50 (5), pp.1725-6. ⟨10.1128/JCM.06531-11⟩. ⟨hal-00965419⟩