Skip to Main content Skip to Navigation
Journal articles

Computational model combined with in vitro experiments to analyse mechanotransduction during mesenchymal STEM cell adhesion

Abstract : The shape that stem cells reach at the end of adhesion process influences their differentiation. Rearrangement of cytoskeleton and modification of intracellular tension may activate mechanotransduction pathways controlling cell commitment. In the present study, the mechanical signals involved in cell adhesion were computed in in vitro stem cells of different shapes using a single cell model, the so-called Cytoskeleton Divided Medium (CDM) model. In the CDM model, the filamentous cytoskeleton and nucleoskeleton networks were represented as a mechanical system of multiple tensile and compressive interactions between the nodes of a divided medium. The results showed that intracellular tonus, focal adhesion forces as well as nuclear deformation increased with cell spreading. The cell model was also implemented to simulate the adhesion process of a cell that spreads on protein-coated substrate by emitting filopodia and creating new distant focal adhesion points. As a result, the cell model predicted cytoskeleton reorganisation and reinforcement during cell spreading. The present model quantitatively computed the evolution of certain elements of mechanotransduction and may be a powerful tool for understanding cell mechanobiology and designing biomaterials with specific surface properties to control cell adhesion and differentiation.
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-00961227
Contributor : Richard BASCHERA Connect in order to contact the contributor
Submitted on : Wednesday, December 15, 2021 - 11:47:25 AM
Last modification on : Saturday, August 6, 2022 - 3:27:26 AM
Long-term archiving on: : Wednesday, March 16, 2022 - 6:38:07 PM

File

2013_Milan2013_ECM.pdf
Explicit agreement for this submission

Identifiers

  • HAL Id : hal-00961227, version 1

Citation

Jean-Louis Milan, Sandrine Lavenus, Paul Pilet, Guy Louarn, Sylvie Wendling, et al.. Computational model combined with in vitro experiments to analyse mechanotransduction during mesenchymal STEM cell adhesion. eCells and Materials Journal, AO Research Institute Davos, 2013, 25, pp.97. ⟨hal-00961227⟩

Share

Metrics

Record views

52

Files downloads

2