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Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope.

Charles-Edouard Leroux 1 Alexei Grichine 2 Irène Wang 3 Antoine Delon 3, *
* Corresponding author
1 MOTIV
Institut Langevin ondes et images
3 MOTIV
LIPhy [2011-2015] - Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] [2011-2015]
Abstract : We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive tool to assess the optical aberrations introduced by the cell. We demonstrate an adaptive optics (AO) system to remove the aberration-related bias in the FFM measurements. Our data show that AO is not only useful when imaging deep in tissues but also when performing FFM measurements through a single cellular layer. This work paves the way for the application of FFM to complex three-dimensional multicellular samples.
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Charles-Edouard Leroux, Alexei Grichine, Irène Wang, Antoine Delon. Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope.. Optics Letters, Optical Society of America - OSA Publishing, 2013, 38 (14), pp.2401-3. ⟨hal-00958198⟩

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