Poly(γ-benzyl-l-glutamate)-PEG-alendronate multivalent nanoparticles for bone targeting. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International Journal of Pharmaceutics Année : 2014

Poly(γ-benzyl-l-glutamate)-PEG-alendronate multivalent nanoparticles for bone targeting.

Laura de Miguel
  • Fonction : Auteur
Magali Noiray
Bogdan I Iorga
Gilles Ponchel
  • Fonction : Auteur

Résumé

Hydroxyapatite (HAP), a highly specific component of bone tissue, is the main target in order to impart osteotropicity. Bone targeted nanoparticles can increase the strength of the interaction with HAP through multivalency and thus constitute a valuable strategy in the therapeutics of skeletal diseases. PBLG10k-b-PEG6k-alendronate nanoparticles (∼75nm) were prepared by a simple nanoprecipitation method. The calcium affinity (KCa(+2)=1.8×10(4)M(-1)) of these nanoparticles was evaluated using isothermal titration calorimetry. The multivalent interaction with HAP surfaces (KHAP) was studied by fluorescence and was estimated to be 1.1×10(10)M(-1), which is more than 4000 times stronger than the reported monovalent interaction between alendronate and HAP surfaces. Molecular modeling suggests that the number of binding sites available at the HAP surface is in large excess than what is required for the whole surface coverage by alendronate decorated nanoparticles. The lower calcium affinity of these nanoparticles than for HAP allows calcium bound nanoparticles to interact with HAP, which yields a deeper understanding of bone targeted carriers and could potentially improve their bone targeting properties.

Domaines

Chimie organique
Fichier non déposé

Dates et versions

hal-00945856 , version 1 (13-02-2014)

Identifiants

Citer

Laura de Miguel, Magali Noiray, Georgiana Surpateanu, Bogdan I Iorga, Gilles Ponchel. Poly(γ-benzyl-l-glutamate)-PEG-alendronate multivalent nanoparticles for bone targeting.. International Journal of Pharmaceutics, 2014, 460 (1-2), pp.73-82. ⟨10.1016/j.ijpharm.2013.10.048⟩. ⟨hal-00945856⟩
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