Photodissociation and dissociative photoionization mass spectrometry of proteins and noncovalent protein-ligand complexes.

Francis Canon (1) , Aleksandar R A. R. Milosavljević , Guillaume G. van Der Rest , Matthieu M. Réfrégiers , Laurent L. Nahon , Pascale Manchado-Sarni (2) , Veronique V. Cheynier (2) , Alexandre A. Giuliani

1 CSGA - Centre des Sciences du Goût et de l'Alimentation [Dijon]
2 SPO - Sciences Pour l'Oenologie

Weight of evidence: Using synchrotron radiation, photo-fragmentation of an intrinsically disordered protein is probed and compared with classical tandem mass-spectrometry activation techniques. It provides excellent sequence coverage allowing the identification of the protein noncovalent binding sites.

Mots clés: VUV photon activation; dissociative photoionization; mass spectrometry; proteins; tannins

Domaines: Alimentation et Nutrition

Francis Canon, Aleksandar R A. R. Milosavljević, Guillaume G. van Der Rest, Matthieu M. Réfrégiers, Laurent L. Nahon, et al.. Photodissociation and dissociative photoionization mass spectrometry of proteins and noncovalent protein-ligand complexes.. Angewandte Chemie International Edition, 2013, 52 (32), pp.8377-81. ⟨10.1002/anie.201304046⟩. ⟨hal-00939839⟩