Microconductometric immunosensor for label-free and sensitive detection of Gram-negative bacteria.

Abstract : : Blood safety is a global health goal. In developed countries, bacterial contamination of platelet concentrates is the highest infectious risk in transfusion despite the current preventive strategies. We aimed to develop a conductometric biosensor for the generic, rapid and sensitive detection of Gram-negative bacteria. Our strategy is based on immunosensors: addressable magnetic nanoparticles coupled with anti-LPS antibodies were used for the generic capture of Gram-negative bacteria. Bacterial capture was characterized by impedancemetric and microscopic measurements. The results obtained with conductometric measurements allowed real-time, sensitive detection of Escherichia coli or Serratia marcescens cultures from 1 to 10(3)CFUmL(-1). The ability of the immunosensor to detect Gram negative bacteria was also tested on clinically relevant strains. The conductometric immunosensor allowed the direct detection of 10-10(3)CFUmL(-1) of Pseudomonas aeruginosa and Acinetobacter baumannii strains that were undetectable using standard immunoblot methods. Results showed that the conductometric response was not inhibited in 1% serum.
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Submitted on : Friday, December 6, 2013 - 3:49:31 PM
Last modification on : Thursday, February 8, 2018 - 11:10:21 AM

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Sarra El Ichi, Fanny Leon, Ludivine Vossier, Hélène Marchandin, Abdelhamid Errachid, et al.. Microconductometric immunosensor for label-free and sensitive detection of Gram-negative bacteria.. Biosensors and Bioelectronics, Elsevier, 2014, 54, pp.378-384. 〈10.1016/j.bios.2013.11.016〉. 〈hal-00915124〉

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