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High sensitive mesoporous TiO2-coated Love wave device for heavy metal detection

Abstract : This work deals with the design of a highly sensitive whole cell-based biosensor for heavy metal detection in liquid medium. The biosensor is constituted of a Love wave sensor coated with a polyelectrolyte multilayer (PEM). Escherichia Coli bacteria are used as bioreceptors as their viscoelastic properties are influenced by toxic heavy metals. The acoustic sensor is constituted of a quartz substrate with interdigitated transducers and a SiO2 guiding layer. However, SiO2 shows some degradation when used in a saline medium. Mesoporous TiO2 presents good mechanical and chemical stability and offers a high active surface area. Then, the addition of a thin titania layer dip-coated onto the acoustic path of the sensor is proposed to overcome the silica degradation and to improve the mass effect sensitivity of the acoustic device. \{PEM\} and bacteria deposition, and heavy metal influence, are real time monitored through the resonance frequency variations of the acoustic device. The first polyelectrolyte layer is inserted through the titania mesoporosity, favouring rigid link of the \{PEM\} on the sensor and improving the device sensitivity. Also, the mesoporosity of surface increases the specific surface area which can be occupied and favors the formation of homogeneous PEM. It was found a frequency shift near 20±1kHz for bacteria immobilization with titania film instead of 7±3kHz with bare silica surface. The sensitivity is highlighted towards cadmium detection. Moreover, in this paper, particular attention is given to the immobilization of bacteria and to biosensor lifetime. Atomic Force Microscopy characterizations of the biosurface have been done for several weeks. They showed significant morphological differences depending on the bacterial life time. We noticed that the lifetime of the biosensor is longer in the case of using a mesoporous TiO2 layer.
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Submitted on : Wednesday, February 25, 2015 - 4:05:58 PM
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Ibtissem Gammoudi, Laurianne Blanc, Fabien Moroté, Christine Grauby-Heywang, Cédric Boissière, et al.. High sensitive mesoporous TiO2-coated Love wave device for heavy metal detection. Biosensors and Bioelectronics, Elsevier, 2014, 57, pp.162-170. ⟨10.1016/j.bios.2013.12.024⟩. ⟨hal-00914710⟩



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