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Article Dans Une Revue Korean Journal of Chemical Engineering Année : 2013

Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations.

Résumé

Bioactive glass 46S6 and biodegradable therapeutic polymer (Chitosan: CH) have been elaborated to form 46S6-CH composite by freeze-drying process. The kinetics of chemical reactivity and bioactivity at the surface were investigated by using physicochemical techniques, particularly solid-state MAS-NMR. Immortalized cell line used to construct multicellular spheroids was employed as three-dimensional (3D) cell cultures for in vitro studies. Obtained results showed a novel structure of the composite; the chemical treatment (ultrasound, magnetic stirring, freeze drying process and lyophilization) led the bioactive glass particles to be loaded in the chitosan-based materials. 29Si and 31P MAS-NMR results showed the emergence of two new species, QSi 3(OH) and QSi 4, which are characteristic of the vitreous network dissolution in simulated body fluid (SBF). MAS-NMR also confirmed the formation of amorphous calcium phosphate (ACP) at the surface of the initial 46S6-CH. Three-dimensional (3D) cell cultures highlighted the effect of chitosan, where the cell viability reached up to 78% in 46S6-CH composite and up to 67% in 46S6. The association of (CH) and bioactive glass (BG) matrix promotes a highly significant bioactivity, demonstrating surface bone formation and satisfactory behavior in biological environment.

Dates et versions

hal-00914224 , version 1 (05-12-2013)

Identifiants

Citer

Hassane Oudadesse, Eric Wers, Xuang Vuong Bui, Claire Roiland, Bruno Bureau, et al.. Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations.. Korean Journal of Chemical Engineering, 2013, 30 (9), pp.1775-1783. ⟨10.1007/s11814-013-0104-x⟩. ⟨hal-00914224⟩
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