Measuring the scattering coefficient of turbid media from two-photon microscopy

David Sevrain (1) , Matthieu Dubreuil (1) , Aymeric Leray (2) , Christophe Odin (3) , Yann Le Grand (1)

1 LSOL - Laboratoire de Spectrométrie et Optique Laser
2 IRI - Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq]
3 IPR - Institut de Physique de Rennes

In this paper, we propose a new and simple method based on two-photon excitation fluorescence (TPEF) microscopy to measure the scattering coefficient μs of thick turbid media. We show, from Monte Carlo simulations, that μs can be derived from the axial profile of the ratio of the TPEF signals epi-collected by the confocal and the non-descanned ports of a scanning microscope, independently of the anisotropy factor g and of the absorption coefficient μa of the medium. The method is validated experimentally on tissue-mimicking optical phantoms, and is shown to have potential for imaging the scattering coefficient of heterogeneous media

Mots clés: microscopy; scattering; two-photon emission fluorescence

Domaines: Optique [physics.optics]

David Sevrain, Matthieu Dubreuil, Aymeric Leray, Christophe Odin, Yann Le Grand. Measuring the scattering coefficient of turbid media from two-photon microscopy. Optics Express, 2013, 21 (21), pp.25221. ⟨10.1364/OE.21.025221⟩. ⟨hal-00907898⟩