Dissociation Kinetics of a Binary Complex in Solution by Protein Displacement.

Helene Launay (1, 2) , Benjamin Parent (3) , Adeline Page (4) , Xavier Hanoulle (1, 2) , Guy Lippens (1, 2)

1 UGSF - Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576
2 Université Lille Nord de France (COMUE)
3 ISEN-Lille - Institut Supérieur de l'Electronique et du Numérique - Lille
4 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire

: Kinetics determined by NMR: Whereas most measurements of kinetic parameters of a ligand-protein complex use displacement of the ligand, protein displacement can give accurate off-rates in solution. Analytical and simulation results are given for the apparent off-rate obtained by protein displacement.

Domaines: Biochimie [q-bio.BM]

Helene Launay, Benjamin Parent, Adeline Page, Xavier Hanoulle, Guy Lippens. Dissociation Kinetics of a Binary Complex in Solution by Protein Displacement.. Angewandte Chemie International Edition, 2013, epub ahead of print. ⟨10.1002/anie.201306253⟩. ⟨hal-00903363⟩