Involvement of equine chorionic gonadotropin (eCG) carbohydrate side chains in its bioactivity; lessons from recombinant hormone expressed in insect cells

Sébastien Legardinier , Claire Cahoreau , Danièle Klett , Yves Combarnous

Natural eCG consists of as much as 45% carbohydrate side chains. The present paper deals with the analysis of the roles of the N- and O-linked saccharides of this hormone in the different steps of its activity and its possible repacement by recombinant eCG expressed in baculovirus - insect cell systems.

Mots clés: half-life; gonadotropin; carbohydrate; recombinant hormone; baculovirus

Domaines: Biologie de la reproduction; Alimentation et Nutrition; Biologie du développement

Sébastien Legardinier, Claire Cahoreau, Danièle Klett, Yves Combarnous. Involvement of equine chorionic gonadotropin (eCG) carbohydrate side chains in its bioactivity; lessons from recombinant hormone expressed in insect cells. Reproduction Nutrition Development, 2005, 45 (3), pp.255-259. ⟨10.1051/rnd:2005018⟩. ⟨hal-00900558⟩