Acoustic, electrochemical and microscopic characterization of interaction of Arthrospira platensis biofilm and heavy metal ions

Abstract : This study examines a biofilm of Arthrospira platensis and its interactions with cadmium and mercury, using electrochemical admittance spectroscopy technique combined with highly sensitive Love wave platform for the real-time detection in liquid medium. Spirulina cells were immobilized via multilayers of polyelectrolyte (PEM) on Si/SiO2 surface of both transducers and characterized using atomic force microscopy (AFM). Scanning electron microscopy (SEM) cell images revealed a first defense mechanism against cadmium at 10−12 M and it immediately takes place after 4 s from injection. The cyanobacteria biofilm becomes more conductive, due to an increase of polyphosphate bodies. An increase of density induces a decrease of frequency. Response time τ90% of the biofilm toward Cd2+ was between 6 and 8 min, while it did not exceed a few seconds toward Hg2+ at 10−12 M. However, the initial rapid stage of mercury adsorption took 40 s to reach the saturated stage. Once external sorption reached the saturated stage, internal mercury uptake began; cations were transported across the cell membrane into the cytoplasm and a beta-HgS precipitation took place, inducing conductivity biofilm decrease, and generating an increase of density, and thus a frequency decrease. SEM images revealed the beginning cell damage at 10−06 M of cadmium and mercury.
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Nadèje Tekaya, Ibtissèm Gammoudi, Mohamed Braiek, Hakim Tarbague, Fabien Morote, et al.. Acoustic, electrochemical and microscopic characterization of interaction of Arthrospira platensis biofilm and heavy metal ions. Journal of Environmental Chemical Engineering, Elsevier, 2013, 1 (3), pp.609-619. ⟨10.1016/j.jece.2013.07.006⟩. ⟨hal-00878629⟩

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