Skip to Main content Skip to Navigation
Journal articles

Pathogenic Mouse Hepatitis Virus or Poly(I:C) Induce IL-33 in Hepatocytes in Murine Models of Hepatitis.

Abstract : The IL-33/ST2 axis is known to be involved in liver pathologies. Although, the IL-33 levels increased in sera of viral hepatitis patients in human, the cellular sources of IL-33 in viral hepatitis remained obscure. Therefore, we aimed to investigate the expression of IL-33 in murine fulminant hepatitis induced by a Toll like receptor (TLR3) viral mimetic, poly(I:C) or by pathogenic mouse hepatitis virus (L2-MHV3). The administration of poly(I:C) plus D-galactosamine (D-GalN) in mice led to acute liver injury associated with the induction of IL-33 expression in liver sinusoidal endothelial cells (LSEC) and vascular endothelial cells (VEC), while the administration of poly(I:C) alone led to hepatocyte specific IL-33 expression in addition to vascular IL-33 expression. The hepatocyte-specific IL-33 expression was down-regulated in NK-depleted poly(I:C) treated mice suggesting a partial regulation of IL-33 by NK cells. The CD1d KO (NKT deficient) mice showed hepatoprotection against poly(I:C)-induced hepatitis in association with increased number of IL-33 expressing hepatocytes in CD1d KO mice than WT controls. These results suggest that hepatocyte-specific IL-33 expression in poly(I:C) induced liver injury was partially dependent of NK cells and with limited role of NKT cells. In parallel, the L2-MHV3 infection in mice induced fulminant hepatitis associated with up-regulated IL-33 expression as well as pro-inflammatory cytokine microenvironment in liver. The LSEC and VEC expressed inducible expression of IL-33 following L2-MHV3 infection but the hepatocyte-specific IL-33 expression was only evident between 24 to 32h of post infection. In conclusion, the alarmin cytokine IL-33 was over-expressed during fulminant hepatitis in mice with LSEC, VEC and hepatocytes as potential sources of IL-33.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-00874120
Contributor : Morgane Le Corre <>
Submitted on : Thursday, October 17, 2013 - 11:49:54 AM
Last modification on : Thursday, January 14, 2021 - 11:20:12 AM
Long-term archiving on: : Saturday, January 18, 2014 - 4:24:06 AM

File

Pathogenic_Mouse_Hepatitis-acc...
Publisher files allowed on an open archive

Identifiers

Citation

Muhammad Imran Arshad, Solène Patrat-Delon, Claire Piquet-Pellorce, Annie l'Helgoualc'H, Michel Rauch, et al.. Pathogenic Mouse Hepatitis Virus or Poly(I:C) Induce IL-33 in Hepatocytes in Murine Models of Hepatitis.. PLoS ONE, Public Library of Science, 2013, 8 (9), pp.e74278. ⟨10.1371/journal.pone.0074278⟩. ⟨hal-00874120⟩

Share

Metrics

Record views

533

Files downloads

1071