The Arabidopsis peptide kiss of death is an inducer of programmed cell death

Abstract : Programmed cell death (PCD) has a key role in defence and development of all multicellular organisms. In plants, there is a large gap in our knowledge of the molecular machinery involved at the various stages of PCD, especially the early steps. Here, we identify kiss of death (KOD) encoding a 25-amino-acid peptide that activates a PCD pathway in Arabidopsis thaliana. Two mutant alleles of KOD exhibited a reduced PCD of the suspensor, a single file of cells that support embryo development, and a reduced PCD of root hairs after a 55 degrees C heat shock. KOD expression was found to be inducible by biotic and abiotic stresses. Furthermore, KOD expression was sufficient to cause death in leaves or seedlings and to activate caspase-like activities. In addition, KOD-induced PCD required light in leaves and was repressed by the PCD-suppressor genes AtBax inhibitor 1 and p35. KOD expression resulted in depolarization of the mitochondrial membrane, placing KOD above mitochondria dysfunction, an early step in plant PCD. A KOD::GFP fusion, however, localized in the cytosol of cells and not mitochondria
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Submitted on : Wednesday, October 16, 2013 - 3:39:55 PM
Last modification on : Tuesday, April 16, 2019 - 6:13:39 PM

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Robert Blanvillain, Bennett Young, Yao-Min Cai, Valérie Hecht, Fabrice Varoquaux, et al.. The Arabidopsis peptide kiss of death is an inducer of programmed cell death. EMBO Journal, EMBO Press, 2011, 30 (6), pp.1173-1183. ⟨10.1038/emboj.2011.14⟩. ⟨hal-00873858⟩

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