A map of cell type-specific auxin responses.

Bastiaan O R Bargmann (1, 2) , Steffen Vanneste (3, 4) , Gabriel Krouk (5) , Tal Nawy (1) , Idan Efroni (1) , Eilon Shani (2) , Goh Choe (2) , Jiří Friml (3, 4, 6) , Dominique C Bergmann (7) , Mark Estelle (2) , Kenneth D Birnbaum (1)

1 Center for Genomics and Systems Biology
2 UCSD - Department of Cell and Developmental Biology
3 Department of Plant Biotechnology and Genetics [Ghent]
4 Department of Plant Biotechnology and Bioinformatics
5 BPMP - Biochimie et Physiologie Moléculaire des Plantes
6 IST Austria - Institute of Science and Technology [Klosterneuburg, Austria]
7 HHMI - Howard Hughes Medical Institute

In plants, changes in local auxin concentrations can trigger a range of developmental processes as distinct tissues respond differently to the same auxin stimulus. However, little is known about how auxin is interpreted by individual cell types. We performed a transcriptomic analysis of responses to auxin within four distinct tissues of the Arabidopsis thaliana root and demonstrate that different cell types show competence for discrete responses. The majority of auxin-responsive genes displayed a spatial bias in their induction or repression. The novel data set was used to examine how auxin influences tissue-specific transcriptional regulation of cell-identity markers. Additionally, the data were used in combination with spatial expression maps of the root to plot a transcriptomic auxin-response gradient across the apical and basal meristem. The readout revealed a strong correlation for thousands of genes between the relative response to auxin and expression along the longitudinal axis of the root. This data set and comparative analysis provide a transcriptome-level spatial breakdown of the response to auxin within an organ where this hormone mediates many aspects of development.

Mots clés: root apical meristem; signaling gradient; development; Arabidopsis

Domaines: Botanique

Bastiaan O R Bargmann, Steffen Vanneste, Gabriel Krouk, Tal Nawy, Idan Efroni, et al.. A map of cell type-specific auxin responses.. Molecular Systems Biology, 2013, 9, pp.688. ⟨10.1038/msb.2013.40⟩. ⟨hal-00873845⟩