Ultrafast carbonyl motion of the Photoactive Yellow Protein chromophore probed by femtosecond circular dichroism

Lucille Mendonça (1) , François Hache (1) , Pascale Changenet-Barret (2) , Pascal Plaza (3) , Haik Chosrowjan (4) , Seiji Taniguchi (4) , Yasushi Imamoto (5)

1 LOB - Laboratoire d'optique et biosciences
2 LFP - URA 2453 - Laboratoire Francis PERRIN
3 PASTEUR - Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640)
4 ILT - Institute for Laser Technology
5 Department of Biophysics

Motions of the trans-p-coumaric acid carbonyl group following the photoexcitation of the R52Q mutant of Photoactive Yellow Protein (PYP) are investigated, for the first time, by ultrafast time-resolved circular dichroism (TRCD) spectroscopy. TRCD is monitored in the near ultraviolet, over a timescale of 10 ps. Immediately after excitation, TRCD is found to exhibit a large negative peak, which decays within a few picoseconds. A quantitative analysis of the signals shows that, upon excitation, the carbonyl group undergoes a fast (<

Mots clés: Circular dichroism; photoactive yellow protein; femtosecond spectroscopy; carbonyl; pump-probe; R52Q

Domaines: Chimie théorique et/ou physique

Lucille Mendonça, François Hache, Pascale Changenet-Barret, Pascal Plaza, Haik Chosrowjan, et al.. Ultrafast carbonyl motion of the Photoactive Yellow Protein chromophore probed by femtosecond circular dichroism. Journal of the American Chemical Society, 2013, 135, pp.14637. ⟨10.1021/ja404503q⟩. ⟨hal-00871078⟩