Ultrafast carbonyl motion of the Photoactive Yellow Protein chromophore probed by femtosecond circular dichroism

Abstract : Motions of the trans-p-coumaric acid carbonyl group following the photoexcitation of the R52Q mutant of Photoactive Yellow Protein (PYP) are investigated, for the first time, by ultrafast time-resolved circular dichroism (TRCD) spectroscopy. TRCD is monitored in the near ultraviolet, over a timescale of 10 ps. Immediately after excitation, TRCD is found to exhibit a large negative peak, which decays within a few picoseconds. A quantitative analysis of the signals shows that, upon excitation, the carbonyl group undergoes a fast (<
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https://hal.archives-ouvertes.fr/hal-00871078
Contributor : Pascal Plaza <>
Submitted on : Tuesday, October 8, 2013 - 5:07:40 PM
Last modification on : Thursday, March 21, 2019 - 1:02:07 PM

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Lucille Mendonça, François Hache, Pascale Changenet-Barret, Pascal Plaza, Haik Chosrowjan, et al.. Ultrafast carbonyl motion of the Photoactive Yellow Protein chromophore probed by femtosecond circular dichroism. Journal of the American Chemical Society, American Chemical Society, 2013, 135, pp.14637. ⟨10.1021/ja404503q⟩. ⟨hal-00871078⟩

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