D. E. Clapham, Calcium signaling, Cell, vol.131, pp.1047-1058, 2007.

S. Feske, Calcium signalling in lymphocyte activation and disease, Nat Rev Immunol, vol.7, pp.690-702, 2007.

S. Feske, Y. Gwack, M. Prakriya, S. Srikanth, and S. H. Puppel, A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function, Nature, vol.441, pp.179-185, 2006.

R. S. Lewis, The molecular choreography of a store-operated calcium channel, Nature, vol.446, pp.284-287, 2007.

R. S. Lewis, Calcium signaling mechanisms in T lymphocytes, Annu Rev Immunol, vol.19, pp.497-521, 2001.

C. Randriamampita, G. Boulla, P. Revy, F. Lemaitre, and A. Trautmann, T cell adhesion lowers the threshold for antigen detection, Eur J Immunol, vol.33, pp.1215-1223, 2003.

R. E. Dolmetsch and R. S. Lewis, Signaling between intracellular Ca2+ stores and depletion-activated Ca2+ channels generates [Ca2+]i oscillations in T lymphocytes, J Gen Physiol, vol.103, pp.365-388, 1994.

N. R. Bhakta and R. S. Lewis, Real-time measurement of signaling and motility during T cell development in the thymus, Semin Immunol, vol.17, pp.411-420, 2005.

N. R. Bhakta, D. Y. Oh, and R. S. Lewis, Calcium oscillations regulate thymocyte motility during positive selection in the three-dimensional thymic environment, Nat Immunol, vol.6, pp.143-151, 2005.

E. Donnadieu, G. Bismuth, and A. Trautmann, Antigen recognition by helper T cells elicits a sequence of distinct changes of their shape and intracellular calcium, Curr Biol, vol.4, pp.584-595, 1994.

E. Donnadieu, G. Bismuth, and A. Trautmann, The intracellular Ca2+ concentration optimal for T cell activation is quite different after ionomycin or CD3 stimulation, Pflugers Arch, vol.429, pp.546-554, 1995.

J. Delon, N. Bercovici, R. Liblau, and A. Trautmann, Imaging antigen recognition by naive CD4+ T cells: compulsory cytoskeletal alterations for the triggering of an intracellular calcium response, Eur J Immunol, vol.28, pp.716-729, 1998.

J. Delon, N. Bercovici, G. Raposo, R. Liblau, and A. Trautmann, Antigendependent and-independent Ca2+ responses triggered in T cells by dendritic cells compared with B cells, J Exp Med, vol.188, pp.1473-1484, 1998.

E. Donnadieu, P. Revy, and A. Trautmann, Imaging T-cell antigen recognition and comparing immunological and neuronal synapses, Immunology, vol.103, pp.417-425, 2001.

P. A. Reay, K. Matsui, K. Haase, C. Wulfing, and Y. H. Chien, Determination of the relationship between T cell responsiveness and the number of MHC-peptide complexes using specific monoclonal antibodies, J Immunol, vol.164, pp.5626-5634, 2000.

J. Huang, V. I. Zarnitsyna, B. Liu, L. J. Edwards, and N. Jiang, The kinetics of two-dimensional TCR and pMHC interactions determine T-cell responsiveness, Nature, vol.464, pp.932-936, 2010.

J. B. Huppa, M. Axmann, M. A. Mortelmaier, B. F. Lillemeier, and E. W. Newell, TCR-peptide-MHC interactions in situ show accelerated kinetics and increased affinity, Nature, vol.463, pp.963-967, 2010.

D. J. Irvine, M. A. Purbhoo, M. Krogsgaard, and M. M. Davis, Direct observation of ligand recognition by T cells, Nature, vol.419, pp.845-849, 2002.

M. Huse, L. O. Klein, A. T. Girvin, J. M. Faraj, and Q. J. Li, Spatial and temporal dynamics of T cell receptor signaling with a photoactivatable agonist, Immunity, vol.27, pp.76-88, 2007.

J. B. Imboden and J. D. Stobo, Transmembrane signalling by the T cell antigen receptor. Perturbation of the T3-antigen receptor complex generates inositol phosphates and releases calcium ions from intracellular stores, J Exp Med, vol.161, pp.446-456, 1985.

E. C. Schwarz, C. Kummerow, A. S. Wenning, K. Wagner, and A. Sappok, Calcium dependence of T cell proliferation following focal stimulation, Eur J Immunol, vol.37, pp.2723-2733, 2007.

M. Thiel, M. J. Wolfs, S. Bauer, A. S. Wenning, and T. Burckhart, Efficiency of T-cell costimulation by CD80 and CD86 cross-linking correlates with calcium entry, Immunology, vol.129, pp.28-40, 2010.

A. Truneh, F. Albert, P. Golstein, and A. M. Schmitt-verhulst, Early steps of lymphocyte activation bypassed by synergy between calcium ionophores and phorbol ester, Nature, vol.313, pp.318-320, 1985.
DOI : 10.1038/313318a0

E. Oancea and T. Meyer, Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals, Cell, vol.95, pp.307-318, 1998.
DOI : 10.1016/s0092-8674(00)81763-8
URL : https://doi.org/10.1016/s0092-8674(00)81763-8

R. E. Dolmetsch, K. Xu, and R. S. Lewis, Calcium oscillations increase the efficiency and specificity of gene expression, Nature, vol.392, pp.933-936, 1998.

C. Utzny, M. Faroudi, and S. Valitutti, Frequency encoding of T-cell receptor engagement dynamics in calcium time series, Biophys J, vol.88, pp.1-14, 2005.

F. Klauschen, M. Ishii, H. Qi, M. Bajenoff, and J. G. Egen, Quantifying cellular interaction dynamics in 3D fluorescence microscopy data, Nat Protoc, vol.4, pp.1305-1311, 2009.
DOI : 10.1038/nprot.2009.129
URL : https://hal.archives-ouvertes.fr/hal-00432039

S. H. Wei, O. Safrina, Y. Yu, K. R. Garrod, and M. D. Cahalan, Ca2+ signals in CD4+ T cells during early contacts with antigen-bearing dendritic cells in lymph node, J Immunol, vol.179, pp.1586-1594, 2007.

P. A. Negulescu, T. B. Krasieva, A. Khan, H. H. Kerschbaum, and M. D. Cahalan, Polarity of T cell shape, motility, and sensitivity to antigen, Immunity, vol.4, pp.421-430, 1996.
DOI : 10.1016/s1074-7613(00)80409-4
URL : https://doi.org/10.1016/s1074-7613(00)80409-4

A. Boissonnas, L. Fetler, I. S. Zeelenberg, S. Hugues, and S. Amigorena, In vivo imaging of cytotoxic T cell infiltration and elimination of a solid tumor, J Exp Med, vol.204, pp.345-356, 2007.

T. Schroeder, Long-term single-cell imaging of mammalian stem cells, Nat Meth, vol.8, pp.30-35, 2011.
DOI : 10.1038/nmeth.1577

A. Serge, N. Bertaux, H. Rigneault, and D. Marguet, Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes, Nat Methods, vol.5, pp.687-694, 2008.
URL : https://hal.archives-ouvertes.fr/hal-00295014

E. Baus, J. Urbain, O. Leo, and F. Andris, Flow cytometric measurement of calcium influx in murine T cell hybrids using Fluo-3 and an organic-anion transport inhibitor, J Immunol Methods, vol.173, pp.41-47, 1994.

D. Thomas, S. C. Tovey, T. J. Collins, M. D. Bootman, and M. J. Berridge, A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals, Cell Calcium, vol.28, pp.213-223, 2000.

A. Quintana and M. Hoth, Apparent cytosolic calcium gradients in Tlymphocytes due to fura-2 accumulation in mitochondria, Cell Calcium, vol.36, pp.99-109, 2004.
DOI : 10.1016/j.ceca.2004.01.003

X. Li, I. Llorente, and M. Brasch, Improvements in live cell analysis of G protein coupled receptors using second generation BD calcium assay kits, Curr Chem Genomics, vol.2, pp.10-15, 2008.

P. Lipp and E. Niggli, Ratiometric confocal Ca(2+)-measurements with visible wavelength indicators in isolated cardiac myocytes, Cell Calcium, vol.14, pp.359-372, 1993.
DOI : 10.1016/0143-4160(93)90040-d

L. B. Dustin, Ratiometric analysis of calcium mobilization, Clinical and Applied Immunology Reviews, vol.1, pp.5-15, 2000.
DOI : 10.1016/s1529-1049(00)00002-7

A. Quintana, C. Kummerow, C. Junker, U. Becherer, and M. Hoth, Morphological changes of T cells following formation of the immunological synapse modulate intracellular calcium signals, Cell Calcium, vol.45, pp.109-122, 2009.

G. Grynkiewicz, M. Poenie, and R. Y. Tsien, A new generation of Ca2+ indicators with greatly improved fluorescence properties, J Biol Chem, vol.260, pp.3440-3450, 1985.

K. R. Gee, K. A. Brown, W. N. Chen, J. Bishop-stewart, and D. Gray, Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes, Cell Calcium, vol.27, pp.97-106, 2000.
DOI : 10.1054/ceca.1999.0095

N. G. Agrawal and J. J. Linderman, Calcium response of helper T lymphocytes to antigen-presenting cells in a single-cell assay, Biophys J, vol.69, pp.1178-1190, 1995.

P. E. Ross, G. R. Ehring, and M. D. Cahalan, Dynamics of ATP-induced calcium signaling in single mouse thymocytes, J Cell Biol, vol.138, pp.987-998, 1997.

A. Sacan, H. Ferhatosmanoglu, and H. Coskun, CellTrack: an open-source software for cell tracking and motility analysis, Bioinformatics, vol.24, pp.1647-1649, 2008.

B. N. Manz, B. L. Jackson, R. S. Petit, M. L. Dustin, and J. Groves, T-cell triggering thresholds are modulated by the number of antigen within individual T-cell receptor clusters, Proc Natl Acad Sci U S A, vol.108, pp.9089-9094, 2011.

R. T. Carson, K. M. Vignali, D. L. Woodland, and D. A. Vignali, T cell receptor recognition of MHC class II-bound peptide flanking residues enhances immunogenicity and results in altered TCR V region usage, Immunity, vol.7, pp.387-399, 1997.

C. Wulfing, J. D. Rabinowitz, C. Beeson, M. D. Sjaastad, and H. M. Mcconnell, Kinetics and extent of T cell activation as measured with the calcium signal, J Exp Med, vol.185, pp.1815-1825, 1997.

H. Gouy, D. Cefai, S. B. Christensen, P. Debre, and G. Bismuth, Ca2+ influx in human T lymphocytes is induced independently of inositol phosphate production by mobilization of intracellular Ca2+ stores. A study with the Ca2+ endoplasmic reticulum-ATPase inhibitor thapsigargin, Eur J Immunol, vol.20, pp.2269-2275, 1990.

M. D. Bootman, T. J. Collins, L. Mackenzie, H. L. Roderick, and M. J. Berridge, 2-aminoethoxydiphenyl borate (2-APB) is a reliable blocker of storeoperated Ca2+ entry but an inconsistent inhibitor of InsP3-induced Ca2+ release, FASEB J, vol.16, pp.1145-1150, 2002.

O. Feinerman, J. Veiga, J. R. Dorfman, R. N. Germain, A. et al., Variability and robustness in T cell activation from regulated heterogeneity in protein levels, Science, vol.321, pp.1081-1084, 2008.

M. L. Dustin, S. K. Bromley, Z. Kan, D. A. Peterson, and E. R. Unanue, Antigen receptor engagement delivers a stop signal to migrating T lymphocytes, Proc Natl Acad Sci U S A, vol.94, pp.3909-3913, 1997.

F. Asperti-boursin, E. Real, G. Bismuth, A. Trautmann, and E. Donnadieu, CCR7 ligands control basal T cell motility within lymph node slices in a phosphoinositide 3-kinase-independent manner, J Exp Med, vol.204, pp.1167-1179, 2007.

H. D. Moreau, F. Lemaitre, E. Terriac, G. Azar, and M. Piel, Situ Cytometry Uncovers T Cell Receptor Signaling during Immunological Synapses and Kinapses In Vivo, vol.37, pp.351-63, 2012.
DOI : 10.1016/j.immuni.2012.05.014
URL : https://doi.org/10.1016/j.immuni.2012.05.014

M. J. Miller, S. H. Wei, I. Parker, and M. D. Cahalan, Two-photon imaging of lymphocyte motility and antigen response in intact lymph node, Science, vol.296, pp.1869-1873, 2002.

P. Beemiller, J. Jacobelli, and M. F. Krummel, Integration of the movement of signaling microclusters with cellular motility in immunological synapses, Nat Immunol, vol.13, pp.787-795, 2012.

D. Skokos, G. Shakhar, R. Varma, J. C. Waite, and T. O. Cameron, PeptideMHC potency governs dynamic interactions between T cells and dendritic cells in lymph nodes, Nat Immunol, vol.8, pp.835-844, 2007.

G. A. Azar, F. Lemaitre, E. A. Robey, and P. Bousso, Subcellular dynamics of T cell immunological synapses and kinapses in lymph nodes, Proc Natl Acad Sci U S A, vol.107, pp.3675-3680, 2010.

M. J. Miller, O. Safrina, I. Parker, and M. D. Cahalan, Imaging the single cell dynamics of CD4+ T cell activation by dendritic cells in lymph nodes, J Exp Med, vol.200, pp.847-856, 2004.

R. N. Germain and I. Stefanova, The dynamics of T cell receptor signaling: complex orchestration and the key roles of tempo and cooperation, Annu Rev Immunol, vol.17, pp.467-522, 1999.

R. Wollman and T. Meyer, Coordinated oscillations in cortical actin and Ca(2+) correlate with cycles of vesicle secretion, Nat Cell Biol, vol.14, pp.1261-1269, 2012.

M. Krogsgaard, Q. J. Li, C. Sumen, J. B. Huppa, and M. Huse, Agonist/ endogenous peptide-MHC heterodimers drive T cell activation and sensitivity, Nature, vol.434, pp.238-243, 2005.
DOI : 10.1038/nature03391

P. Revy, M. Sospedra, B. Barbour, and A. Trautmann, Functional antigenindependent synapses formed between T cells and dendritic cells, Nat Immunol, vol.2, pp.925-931, 2001.
DOI : 10.1038/ni713
URL : https://hal.archives-ouvertes.fr/hal-00145482

W. Y. Ho, M. P. Cooke, C. C. Goodnow, and M. M. Davis, Resting and anergic B cells are defective in CD28-dependent costimulation of naive CD4+ T cells, J Exp Med, vol.179, pp.1539-1549, 1994.

S. Tourne, V. Kouskoff, W. Ho, M. Davis, and C. Benoist, Inhibition of thymocyte positive selection by natural MHC: peptide ligands, Eur J Immunol, vol.29, pp.394-402, 1999.

R. S. Friedman, P. Beemiller, C. M. Sorensen, J. Jacobelli, and M. F. Krummel, Real-time analysis of T cell receptors in naive cells in vitro and in vivo reveals flexibility in synapse and signaling dynamics, J Exp Med, vol.207, pp.2733-2749, 2010.

K. D. Omilusik, L. L. Nohara, S. Stanwood, W. Jefferies, W. Weft et al., The Intricate Tapestry of Calcium Channels Regulating T Lymphocyte Function, Front Immunol, vol.4, p.164, 2013.

K. Omilusik, J. J. Priatel, X. Chen, Y. T. Wang, and H. Xu, The Ca(v)1.4 calcium channel is a critical regulator of T cell receptor signaling and naive T cell homeostasis, Immunity, vol.35, pp.349-360, 2011.

B. A. Niemeyer and M. Hoth, Excitable T cells: Ca(v)1.4 channel contributions and controversies, Immunity, vol.35, pp.315-317, 2011.

S. Valitutti, D. Coombs, and L. Dupre, The space and time frames of T cell activation at the immunological synapse, FEBS Lett, vol.584, pp.4851-4857, 2010.

G. Giannone, E. Hosy, F. Levet, A. Constals, and K. Schulze, Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density, Biophys J, vol.99, pp.1303-1310, 2010.
URL : https://hal.archives-ouvertes.fr/hal-00661871

E. Deutsch, A. V. Weigel, E. J. Akin, P. Fox, and G. Hansen, Kv2.1 cell surface clusters are insertion platforms for ion channel delivery to the plasma membrane, Mol Biol Cell, vol.23, pp.2917-2929, 2012.

B. Qu, D. Al-ansary, C. Kummerow, M. Hoth, and E. C. Schwarz, ORAImediated calcium influx in T cell proliferation, apoptosis and tolerance, Cell Calcium, vol.50, pp.261-269, 2011.

A. Rasola and P. Bernardi, Mitochondrial permeability transition in Ca(2+)dependent apoptosis and necrosis, Cell Calcium, vol.50, pp.222-233, 2011.

S. D. Hess, M. Oortgiesen, and M. D. Cahalan, Calcium oscillations in human T and natural killer cells depend upon membrane potential and calcium influx, J Immunol, vol.150, pp.2620-2633, 1993.

J. Soboloff, B. S. Rothberg, M. Madesh, and D. L. Gill, STIM proteins: dynamic calcium signal transducers, Nat Rev Mol Cell Biol, vol.13, pp.549-565, 2012.

M. F. Ritchie, E. Samakai, and J. Soboloff, STIM1 is required for attenuation of PMCA-mediated Ca2+ clearance during T-cell activation, Embo J, vol.31, pp.1123-1133, 2012.

X. Shi, Y. Bi, W. Yang, X. Guo, and Y. Jiang, Ca(2+) regulates T-cell receptor activation by modulating the charge property of lipids, Nature, vol.493, pp.111-116, 2012.

D. A. Vignali and J. L. Strominger, Amino acid residues that flank core peptide epitopes and the extracellular domains of CD4 modulate differential signaling through the T cell receptor, J Exp Med, vol.179, pp.1945-1956, 1994.