Barcoding T Cell Calcium Response Diversity with Methods for Automated and Accurate Analysis of Cell Signals (MAAACS)

Abstract : We introduce a series of experimental procedures enabling sensitive calcium monitoring in T cell populations by confocal video-microscopy. Tracking and post-acquisition analysis was performed using Methods for Automated and Accurate Analysis of Cell Signals (MAAACS), a fully customized program that associates a high throughput tracking algorithm, an intuitive reconnection routine and a statistical platform to provide, at a glance, the calcium barcode of a population of individual T-cells. Combined with a sensitive calcium probe, this method allowed us to unravel the heterogeneity in shape and intensity of the calcium response in T cell populations and especially in naive T cells, which display intracellular calcium oscillations upon stimulation by antigen presenting cells.
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Submitted on : Tuesday, September 18, 2018 - 11:52:06 AM
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Audrey Salles, Cyrille Billaudeau, Arnaud Sergé, Anne-Marie Bernard, Marie-Claire Phelipot, et al.. Barcoding T Cell Calcium Response Diversity with Methods for Automated and Accurate Analysis of Cell Signals (MAAACS). PLoS Computational Biology, Public Library of Science, 2013, 9 (9), pp.e1003245. ⟨10.1371/journal.pcbi.1003245⟩. ⟨hal-00870686⟩

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