Applications of Projection NMR Techniques
Résumé
Among the range of fast methods that were developed for speeding up NMR data acquisition, mostly over the last decade, projection spectroscopy and reduced dimensionality approaches have found significant area of applications in both solution and solid-state NMR studies. In this review, we illustrate the diversity of these applications, notably in the field of biomolecular NMR. Projection spectroscopy plays a remarkable role in the modern protocols for backbone and side-chains assignment for high-throughput structural studies of small globular proteins, or for investigations of macro-molecular systems exhibiting high chemical shift degeneracy. Approaches that exploit projection spectroscopy and reconstruction approaches for structural investigations of small molecules and their complex mixtures, as well as high-resolution studies of materials in the solid-state are also presented.