Solid-phase synthesis of molecularly imprinted nanoparticles for protein recognition.

G. Ambrosini , S. Beyazit , Karsten Haupt (1) , B. Tse Sum Bui (1)

1 GEC - Génie Enzymatique et Cellulaire

A solid-phase synthesis approach to prepare molecularly imprinted polymer nanoparticles (MIP-NPs) specific for trypsin is described. The protein is immobilized on a solid support upon which thermoresponsive MIP-NPs are synthesized. The MIP-NPs are released by a simple temperature change, resulting in synthetic antibody mimics exhibiting high specificity and selectivity for trypsin.

G. Ambrosini, S. Beyazit, Karsten Haupt, B. Tse Sum Bui. Solid-phase synthesis of molecularly imprinted nanoparticles for protein recognition.. Chemical Communications, 2013, 49, pp.6746-6748. ⟨hal-00866386⟩