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Peroxiredoxins and sulfiredoxin at the crossroads of the NO and H2O2 signaling pathways.

Abstract : Peroxiredoxins (Prxs) are a family of peroxidases that maintain thiol homeostasis by catalyzing the reduction of organic hydroperoxides, H2O2, and peroxynitrite. Eukaryotic 2-Cys-Prxs, also referred to as typical Prxs, can be inactivated by oxidation of the catalytic cysteine to sulfinic acid, which may regulate the intracellular messenger function of H2O2. A small redox protein, sulfiredoxin (Srx), has been shown to reduce sulfinylated 2-Cys-Prxs and thus to regenerate active 2-Cys-Prxs. We previously reported that cytokine-induced nitric oxide (NO) intervenes in this pathway by decreasing the level of 2-Cys overoxidation and by upregulating Srx through the activation of the transcription factor nuclear factor erythroid 2-related factor (Nrf2). Here, we describe the methods used to monitor the interplay between NO and H2O2 in the regulation of the Prx/Srx system in immunostimulated macrophages, which produce both reactive oxygen species and NO.
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https://hal.archives-ouvertes.fr/hal-00856898
Contributor : Jocelyne Brunet <>
Submitted on : Monday, September 2, 2013 - 4:19:37 PM
Last modification on : Monday, June 22, 2020 - 12:08:08 PM

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Kahina Abbas, Sylvie Riquier, Jean-Claude Drapier. Peroxiredoxins and sulfiredoxin at the crossroads of the NO and H2O2 signaling pathways.. Methods in Enzymology, Elsevier, 2013, 527, pp.113-28. ⟨10.1016/B978-0-12-405882-8.00006-4⟩. ⟨hal-00856898⟩

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