Biofunctional micellar nanoparticles from peptide-b-polymer chimeras

Charlotte Drappier (1, 2) , Anne-Laure Wirotius (2) , Katell Bathany (3) , Emmanuel Ibarboure (2) , Olivia Condassamy (2) , Elisabeth Garanger (1, 2, 4) , Sébastien Lecommandoux (2, 4)

1 IECB - Institut Européen de Chimie et de Biologie
2 LCPO - Laboratoire de Chimie des Polymères Organiques
3 CBMN - Chimie et Biologie des Membranes et des Nanoobjets
4 Team 3 LCPO : Polymer Self-Assembly & Life Sciences

We describe the precision synthesis and characterization of amphiphilic peptide-b-polymer chimeras encoding, at the molecular level, self-assembly properties and biological functions, presently cell membrane penetrating motifs (Tat peptide). Self-assembled micellar nanoparticles obtained from a series of Tat-b-poly(trimethylene carbonate) (Tat-b-PTMC) chimeras with tunable hydrophilic fractions were finely characterized in order to establish chimera-nanoparticle structure relationships. Well-defined nanoparticles with diameters ranging from 22 to 40 nm could be obtained by direct self-assembly in buffer.

Mots clés: INTRACELLULAR DRUG-DELIVERY; CELL-PENETRATING PEPTIDES; SYNTHETIC-POLYMER; BLOCK-COPOLYMERS; TAT PEPTIDE; PROTEIN; MEMBRANE; SIRNA; HIV-1; SPECTROSCOPY

Domaines: Polymères

Charlotte Drappier, Anne-Laure Wirotius, Katell Bathany, Emmanuel Ibarboure, Olivia Condassamy, et al.. Biofunctional micellar nanoparticles from peptide-b-polymer chimeras. Polymer Chemistry, 2013, 4 (6), pp.2011-2018. ⟨10.1039/c2py21044d⟩. ⟨hal-00846289⟩