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Two-peaked 5-ALA-induced PpIX fluorescence emission spectrum distinguishes glioblastomas from low grade gliomas and infiltrative component of glioblastomas

B. Montcel 1, * L. Mahieu-Williame 2 Xavier Armoiry 3 D. Meyronet 4, 5 Jacques Guyotat 6
* Corresponding author
1 RMN et optique : De la mesure au biomarqueur
CREATIS - Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé
2 PILoT - Plateforme d'Imagerie Multimodale LyonTech
CREATIS - Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé
Abstract : 5-ALA-induced protoporphyrin IX (PpIX) fluorescence enables to guiding in intra-operative surgical glioma resection. However at present, it has yet to be shown that this method is able to identify infiltrative component of glioma. In extracted tumor tissues we measured a two-peaked emission in low grade gliomas and in the infiltrative component of glioblastomas due to multiple photochemical states of PpIX. The second emission peak appearing at 620 nm (shifted by 14 nm from the main peak at 634 nm) limits the sensibility of current methods to measured PpIX concentration. We propose new measured parameters, by taking into consideration the two-peaked emission, to overcome these limitations in sensitivity. These parameters clearly distinguish the solid component of glioblastomas from low grade gliomas and infiltrative component of glioblastomas.
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https://hal.archives-ouvertes.fr/hal-00828529
Contributor : Béatrice Rayet <>
Submitted on : Friday, May 31, 2013 - 11:30:54 AM
Last modification on : Wednesday, July 8, 2020 - 12:44:07 PM

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B. Montcel, L. Mahieu-Williame, Xavier Armoiry, D. Meyronet, Jacques Guyotat. Two-peaked 5-ALA-induced PpIX fluorescence emission spectrum distinguishes glioblastomas from low grade gliomas and infiltrative component of glioblastomas. Biomedical optics express, Optical Society - SOA Publishing, 2013, 4 (4), pp.548-558. ⟨10.1364/BOE.4.000548⟩. ⟨hal-00828529⟩

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