Two-peaked 5-ALA-induced PpIX fluorescence emission spectrum distinguishes glioblastomas from low grade gliomas and infiltrative component of glioblastomas

B. Montcel (1) , L. Mahieu-Williame (2) , Xavier Armoiry (3) , D. Meyronet (4, 5) , Jacques Guyotat (6)

1 RMN et optique : De la mesure au biomarqueur
2 PILoT - Plateforme d'Imagerie Multimodale LyonTech
3 Pharmacy Department/ Cellule Innovation
4 CRNL - Centre de recherche en neurosciences de Lyon - Lyon Neuroscience Research Center
5 Centre de Pathologie et de Neuropathologie Est
6 Service de Neurochirurgie D

5-ALA-induced protoporphyrin IX (PpIX) fluorescence enables to guiding in intra-operative surgical glioma resection. However at present, it has yet to be shown that this method is able to identify infiltrative component of glioma. In extracted tumor tissues we measured a two-peaked emission in low grade gliomas and in the infiltrative component of glioblastomas due to multiple photochemical states of PpIX. The second emission peak appearing at 620 nm (shifted by 14 nm from the main peak at 634 nm) limits the sensibility of current methods to measured PpIX concentration. We propose new measured parameters, by taking into consideration the two-peaked emission, to overcome these limitations in sensitivity. These parameters clearly distinguish the solid component of glioblastomas from low grade gliomas and infiltrative component of glioblastomas.

Domaines: Traitement du signal et de l'image [eess.SP]

B. Montcel, L. Mahieu-Williame, Xavier Armoiry, D. Meyronet, Jacques Guyotat. Two-peaked 5-ALA-induced PpIX fluorescence emission spectrum distinguishes glioblastomas from low grade gliomas and infiltrative component of glioblastomas. Biomedical optics express, 2013, 4 (4), pp.548-558. ⟨10.1364/BOE.4.000548⟩. ⟨hal-00828529⟩