Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity

D.E. Hunt (1) , V. Klepac-Ceraj (1, 2) , S.G. Acinas (1) , S. Bertilsson (1, 3) , Christian Gautier (1, 4) , M.F. Polz (1)

1 CEE - Department of Civil and Environmental Engineering [Cambridge]
2 Department of Microbiology and Molecular Genetics
3 Limnology [Norbyvägen]
4 LOB - Laboratoire d'optique et biosciences

The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample. Copyright Cop. 2006, American Society for Microbiology. All Rights Reserved.

D.E. Hunt, V. Klepac-Ceraj, S.G. Acinas, S. Bertilsson, Christian Gautier, et al.. Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity. Applied and Environmental Microbiology, 2006, 72 (3), pp.2221. ⟨10.1128/AEM.72.3.2221-2225.2006⟩. ⟨hal-00824466⟩