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Journal articles
Blood-feeding and immunogenic Aedes aegypti saliva proteins.
Ladawan Wasinpiyamongkol
1
Sirilaksana Patramool
2
Natthanej Luplertlop
1
Pornapat Surasombatpattana
2
Souleymane Doucoure
3
François Mouchet
3
Martial Séveno
4
Franck Remoue
3
Edith Demettre
4
Jean-Paul Brizard
5
Patrick Jouin
4
David Georges Biron
6, 7
Frédéric Thomas
2
Dorothée Missé
2
Ladawan Wasinpiyamongkol 1
Author
Sirilaksana Patramool 2
Author
Natthanej Luplertlop 1
Author
Pornapat Surasombatpattana 2
Author
Souleymane Doucoure 3
Author
François Mouchet 3
Author
Martial Séveno 4
Author IdHAL : martial-seveno ORCID : https://orcid.org/0000-0002-5000-8735
Franck Remoue 3
Author IdHAL : franck-remoue
Edith Demettre 4
Author
Jean-Paul Brizard 5
Author
Patrick Jouin 4
Author
David Georges Biron 6, 7
Author IdHAL : david-biron ORCID : https://orcid.org/0000-0002-6531-4647
Frédéric Thomas 2
Author
Dorothée Missé 2
Author Employer institution : Institut de Recherche pour le Développement (IRD en Occitanie) IdHAL : dorothee-misse IdRef : https://www.idref.fr/175100683 ORCID : https://orcid.org/0000-0002-6485-3841
1
Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand (Thailand)
2
MIVEGEC - Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (911 Avenue Agropolis BP 64501 34394 Montpellier cedex 5 - France)
- IRD [France-Sud] - Institut de Recherche pour le Développement : 224 (911 avenue Agropolis, BP 64501 34394 Montpellier cedex 5 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5290 (France)
- UM - Université de Montpellier : UMR5290 (163 rue Auguste Broussonnet - 34090 Montpellier - France)
3
EPIPREV - Épidémiologie et prévention : environnement et efficacité des interventions (911 Avenue d'Agropolis BP 64501 34394 Montpellier cedex 5 - France)
4
IGF - Institut de Génomique Fonctionnelle (141, Rue de la Cardonille 34094 Montpellier cedex 5 - France)
- UM - Université de Montpellier (163 rue Auguste Broussonnet - 34090 Montpellier - France)
- UM1 - Université Montpellier 1 (5, boulevard Henri IV - CS 19044 - 34967 Montpellier cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1191 (101, rue de Tolbiac, 75013 Paris - France)
- UM2 - Université Montpellier 2 - Sciences et Techniques (Place Eugène Bataillon - 34095 Montpellier cedex 5 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5203 (France)
5
VALPEDO - Actualisation et valorisation des données pédologiques tropicales et méditerranéennes. Contributions à la recherche, à l'expertise et à l'aide à la gestion des ressources (IRD Montpellier - BP 64501 - 34394 Montpellier Cedex 5 - France)
6
LMGE - Laboratoire Microorganismes : Génome et Environnement (Campus des Cézeaux / 24, avenue des Landais BP 80026 / 63170 Aubière - France)
- UBP - Université Blaise Pascal - Clermont-Ferrand 2 : UMR6023 (34, avenue Carnot - BP 185 / 63006 Clermont-Ferrand cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR6023 (France)
- UdA - Université d'Auvergne - Clermont-Ferrand I : UMR6023 (49, Boulevard François-Mitterrand / CS 60032 / 63001 Clermont-Ferrand Cedex 1 - France)
7
PIAF - Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (INRA Site de Crouël 234, avenue du Brézet 63100 Clermont-Ferrand - France - France)
- UBP - Université Blaise Pascal - Clermont-Ferrand 2 : UMRA547 (34, avenue Carnot - BP 185 / 63006 Clermont-Ferrand cedex - France)
- INRA - Institut National de la Recherche Agronomique : UMRA547 (France)
Abstract : Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugar-fed mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was up-regulated. The immunofluorescence assay shows that there is no disruption of the SG cytoskeletal after the blood meal. We have generated for the first time the 2-DE profiles of immunogenic Ae. aegypti SG BF-related proteins. These new data could contribute to the understanding of the physiological processes that appear during the blood meal.
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Journal articles
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Complete list of metadatas
https://hal.archives-ouvertes.fr/hal-00820653
Contributor : David Biron <>
Submitted on : Monday, May 6, 2013 - 1:07:06 PM
Last modification on : Thursday, December 3, 2020 - 5:00:02 PM
Contributor : David Biron <>
Submitted on : Monday, May 6, 2013 - 1:07:06 PM
Last modification on : Thursday, December 3, 2020 - 5:00:02 PM
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Identifiers
- HAL Id : hal-00820653, version 1
- DOI : 10.1002/pmic.200900626
- PUBMED : 19882664
- PRODINRA : 51487
- WOS : 000278494100001
Collections
CNRS | LMGE | PRES_CLERMONT | PIAF | INRA | AGROPOLIS | BS | B3ESTE | UNIV-MONTPELLIER | INRAE | POLITIQUES-PUBLIQUES
Citation
Ladawan Wasinpiyamongkol, Sirilaksana Patramool, Natthanej Luplertlop, Pornapat Surasombatpattana, Souleymane Doucoure, et al.. Blood-feeding and immunogenic Aedes aegypti saliva proteins.. Proteomics, Wiley-VCH Verlag, 2010, 10 (10), pp.1906-16. ⟨10.1002/pmic.200900626⟩. ⟨hal-00820653⟩
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