Skip to Main content Skip to Navigation
Journal articles

Functional dissection of the apicomplexan glideosome molecular architecture.

Abstract : The glideosome of apicomplexan parasites is an actin- and myosin-based machine located at the pellicle, between the plasma membrane (PM) and inner membrane complex (IMC), that powers parasite motility, migration, and host cell invasion and egress. It is composed of myosin A, its light chain MLC1, and two gliding-associated proteins, GAP50 and GAP45. We identify GAP40, a polytopic protein of the IMC, as an additional glideosome component and show that GAP45 is anchored to the PM and IMC via its N- and C-terminal extremities, respectively. While the C-terminal region of GAP45 recruits MLC1-MyoA to the IMC, the N-terminal acylation and coiled-coil domain preserve pellicle integrity during invasion. GAP45 is essential for gliding, invasion, and egress. The orthologous Plasmodium falciparum GAP45 can fulfill this dual function, as shown by transgenera complementation, whereas the coccidian GAP45 homolog (designated here as) GAP70 specifically recruits the glideosome to the apical cap of the parasite.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-00820623
Contributor : Valérie Polonais <>
Submitted on : Monday, May 6, 2013 - 11:56:24 AM
Last modification on : Sunday, November 24, 2019 - 10:36:02 PM

Links full text

Identifiers

Collections

Citation

Karine Frénal, Valérie Polonais, Jean-Baptiste Marq, Rolf Stratmann, Julien Limenitakis, et al.. Functional dissection of the apicomplexan glideosome molecular architecture.. Cell Host and Microbe, Elsevier, 2010, 8 (4), pp.343-57. ⟨10.1016/j.chom.2010.09.002⟩. ⟨hal-00820623⟩

Share

Metrics

Record views

252