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Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon.

Abstract : The 2 465 177 bp genome of Sulfolobus islandicus LAL14/1, host of the model rudivirus SIRV2, was sequenced. Exhaustive comparative genomic analysis of S. islandicus LAL14/1 and the nine other completely sequenced S. islandicus strains isolated from Iceland, Russia and USA revealed a highly syntenic common core genome of approximately 2 Mb and a long hyperplastic region containing most of the strain-specific genes. In LAL14/1, the latter region is enriched in insertion sequences, CRISPR (clustered regularly interspaced short palindromic repeats), glycosyl transferase genes, toxin-antitoxin genes and MITE (miniature inverted-repeat transposable elements). The tRNA genes of LAL14/1 are preferential targets for the integration of mobile elements but clusters of atypical genes (CAG) are also integrated elsewhere in the genome. LAL14/1 carries five CRISPR loci with 10 per cent of spacers matching perfectly or imperfectly the genomes of archaeal viruses and plasmids found in the Icelandic hot springs. Strikingly, the CRISPR_2 region of LAL14/1 carries an unusually long 1.9 kb spacer interspersed between two repeat regions and displays a high similarity to pING1-like conjugative plasmids. Finally, we have developed a genetic system for S. islandicus LAL14/1 and created ΔpyrEF and ΔCRISPR_1 mutants using double cross-over and pop-in/pop-out approaches, respectively. Thus, LAL14/1 is a promising model to study virus-host interactions and the CRISPR/Cas defence mechanism in Archaea.
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Contributor : Marie-Claude Serre <>
Submitted on : Saturday, May 16, 2020 - 12:48:06 AM
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Carole Jaubert, Chloë Danioux, Jacques Oberto, Diego Cortez, Ariane Bize, et al.. Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon.. Biology Open, Royal Society, 2013, 3 (4), pp.130010. ⟨10.1098/rsob.130010⟩. ⟨hal-00818894⟩



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