Oligonucleotide solid-phase synthesis on fluorescent nanoparticles grafted on controlled pore glass - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue RSC Advances Année : 2012

Oligonucleotide solid-phase synthesis on fluorescent nanoparticles grafted on controlled pore glass

Résumé

Oligonucleotide solid-phase synthesis is now possible on nano-sized particles, thanks to the use of controlled pore glass-nanoparticle assemblies. We succeeded in anchoring silica nanoparticles (NPs) inside the pores of micrometric glass via a reversible covalent binding. The pore diameter must be at least six times the diameter of the nanoparticle in order to maintain efficient synthesis of oligonucleotides in the synthesizer. We demonstrated that the pores protect NP anchoring during DNA synthesis without decreasing the coupling rate of the phosphoramidite synthons. This bottom-up strategy for NP functionalization with DNA results in unprecedented DNA loading efficiency. We also confirmed that the DNA synthesized on the nanoparticle surface was accessible for hybridization with its complementary DNA strand.
Fichier non déposé

Dates et versions

hal-00816504 , version 1 (22-04-2013)

Identifiants

Citer

Gabriel de Crozals, Carole Farre, Grégoire Hantier, Didier Léonard, Christophe A. Marquette, et al.. Oligonucleotide solid-phase synthesis on fluorescent nanoparticles grafted on controlled pore glass. RSC Advances, 2012, 2 (31), pp.11858-11866. ⟨10.1039/c2ra22077f⟩. ⟨hal-00816504⟩
73 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More