[Post-translational regulation of N-glycosylated proteins expression in human intestinal cells in culture].

E. Ogier-Denis , Christian Sapin (1) , C. Bauvy , J. J. Hourri , M. Aubery , G. Trugnan (2, 3, 4) , P. Codogno

1 ACS - Archéologies, Cultures et Sociétés
2 Trafic Membranaire et Signalisation Dans les Cellules Epitheliales
3 UMRS893 - Centre de Recherche Saint-Antoine
4 UPMC - Université Pierre et Marie Curie - Paris 6

HT-29 cells derived from a human colonic adenocarcinoma, can express a typical intestinal differentiation. Undifferentiated HT-29 cells accumulate N-linked glycoproteins substituted with unprocessed carbohydrate chains before to degrade them. Conversely, carbohydrate chains of N-linked glycoproteins are classically processed in differentiated HT-29 cells. The instability of N-linked glycoproteins in undifferentiated HT-29 cells is due to their rapid delivery from the endoplasmic reticulum to a compartment with lysosomal characteristics. This catabolitic pathway involves a bypass of the Golgi apparatus.

Domaines: Chimie organique

E. Ogier-Denis, Christian Sapin, C. Bauvy, J. J. Hourri, M. Aubery, et al.. [Post-translational regulation of N-glycosylated proteins expression in human intestinal cells in culture].. C R Seances Soc Biol Fil, 1991, 185 ((4)), pp.182-9. ⟨hal-00815093⟩