Optical microscopy and spectroscopy of analyte-sensitive functionalized gold nanoparticles in microfluidic systems

Martinus H. V. Werts (1) , Vincent Raimbault (2) , Matthieu Loumaigne (1) , Laurent Griscom (1) , Olivier Français (1) , Julien R. G. Navarro (1) , Anne Débarre (3) , Bruno Le Pioufle (1)

1 SATIE-BIOMIS - Bio-MIcroSystèmes et BioSensors
2 IMS - Laboratoire de l'intégration, du matériau au système
3 LAC - Laboratoire Aimé Cotton

The use of microfluidic devices for the handling and analysis of suspensions of colloidal gold particles is presented. The plasmonic particles are detected via their resonant light scattering (RLS) in a simple and versatile LED-based dark field illumination geometry. RLS enables both microscopic imaging and microspectroscopy. The measurement of the diffusion coefficient of nanoparticles in this type of devices is demonstrated. Nanoparticles are separated from small (free ligand) molecules in a continuous flow process. Reversible aggregation of functionalized particles is detected in microflow by means of RLS, which opens perspectives for the development of microfluidic bioplasmonic detection schemes.

Domaines: Optique [physics.optics]; Chimie analytique

Martinus H. V. Werts, Vincent Raimbault, Matthieu Loumaigne, Laurent Griscom, Olivier Français, et al.. Optical microscopy and spectroscopy of analyte-sensitive functionalized gold nanoparticles in microfluidic systems. Proc. SPIE 2013, 8595, 85950W, Feb 2013, United States. pp.85950W, ⟨10.1117/12.2001608⟩. ⟨hal-00809421v1⟩