FRET detection in the plasma membrane using Total Internal Reflection Fluorescence Lifetime Imaging Microscopy

Pierre Blandin (1, 2) , Sandrine Leveque-Fort (3) , Sandrine Lecart (4) , Frédéric Druon (1) , Patrick Georges (1) , J.C Cossec (5) , M.C. Pottier (5) , Zsolt Lenkei (5)

1 Laboratoire Charles Fabry de l'Institut d'Optique / Elsa
2 PPM - Laboratoire de Photophysique Moléculaire
3 ISMO - Institut des Sciences Moléculaires d'Orsay
4 CPBM - Centre de Photonique Biomédicale
5 Laboratoire de Neurobiologie

We developed a Total Internal Reflection Fluorescence Lifetime Imaging Microscope to perform functional imaging of living cells membranes labeled with FRET couples. Förster Resonance Energy Transfer efficiency can thus be followed with subwavelength axial resolution.

Domaines: Optique [physics.optics]

Pierre Blandin, Sandrine Leveque-Fort, Sandrine Lecart, Frédéric Druon, Patrick Georges, et al.. FRET detection in the plasma membrane using Total Internal Reflection Fluorescence Lifetime Imaging Microscopy. CLEO (Conference on Lasers and Electro-Optics), May 2008, San José, United States. ⟨hal-00808701⟩