Improved chromatographic performances of glycidyl methacrylate anion-exchange monolith for fast nano-ion exchange chromatography

Anthony Bruchet (1, 2) , Vincent Dugas (3) , Clarisse Mariet (2) , Florence Goutelard (2) , Jérôme Randon (3)

1 ISA - Institut des Sciences Analytiques
2 LANIE - Laboratoire de développement Analytique Nucléaire Isotopique et Elémentaire
3 TECHSEP - TECHniques de SEParations (2011-2014)

An efficient and reproducible photopolymerized poly(glycidyl methacrylate-co-ethylene dimethacrylate) was synthesized in Teflon-coated fused-silica capillaries (100 mu m id) and functionalized by reaction of triethylamine with reactive epoxy groups. We report here the successful transfer of a standard polymerization mixture optimized for the thermally initiated synthesis of glycidyl-based monolith to photo-induced polymerization. The monolith obtained after optimization of the photo-initiation conditions was characterized in reverse-phase chromatography evaluating its suitability in terms of efficiency, retention and hydrodynamic permeability. Reproducibility of the photo-induced procedure was satisfactory with RSD below 6% for retention and efficiency and slightly higher for hydrodynamic permeability (12%). The functionalized generic support was then used in nano-ion-exchange chromatography. Efficiencies up to 75 000 plates/m, ion-exchange capacity of 8 nano-equivalents/cm of monolithic column, with a combination of a satisfactory hydrodynamic permeability allowed to perform fast separations of five inorganic anions in <3 min maintaining baseline resolution. The efficiency of the monolith was not retention-dependent, demonstrating its wide range of possible applications for highly retained anions.

Mots clés: CAPILLARY LIQUID-CHROMATOGRAPHY; POROUS POLYMER MONOLITHS; SURFACE PHOTOGRAFTING PROCESS; CO-ETHYLENE DIMETHACRYLATE); AFFINITY-CHROMATOGRAPHY; MACROPOROUS POLYMER; SMALL MOLECULES; POLAR ANALYTES; Chemical functionalization; Fast nano-IEC; Monolith; Photopolymerization; Strong anion exchanger; STATIONARY PHASES; SEPARATION MEDIA

Domaines: Chimie analytique

Anthony Bruchet, Vincent Dugas, Clarisse Mariet, Florence Goutelard, Jérôme Randon. Improved chromatographic performances of glycidyl methacrylate anion-exchange monolith for fast nano-ion exchange chromatography. Journal of Separation Science, 2011, 34 (16-17 Special Issue), pp.2079-2087. ⟨10.1002/jssc.201100180⟩. ⟨hal-00807504⟩