High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection

Y. H. Cho (1) , S. W. Lee (1) , Bruno Le Pioufle (2) , N. Takama (1) , S. Takeuchi (2) , T. Fujii (2) , B. J. Kim (2)

1 IIS - Institute of Industrial Science
2 LIMMS - Laboratory for Integrated Micro Mechatronics Systems

We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.

Domaines: Micro et nanotechnologies/Microélectronique

Y. H. Cho, S. W. Lee, Bruno Le Pioufle, N. Takama, S. Takeuchi, et al.. High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. Transducers '07 & Eurosensors Xxi, Digest of Technical Papers, Vols 1 and 2, 2007, France. pp.U474-U475. ⟨hal-00795167⟩