Development of a Love-wave Immunosensor with Microfluidic Technology to Detect Phages in Dynamic Mode

Abstract : The goal has been to develop an immunosensor based on a Love-wave device and a PDMS microfluidic chip in order to detect potentially pathogenic microorganisms in real time. Therefore, it has been tested with the phage M13 detected through the mouse monoclonal antibody anti-M13 (AM13). The Double-layer Agar (DLA) technique has been used to quantify the number of phages, linked to the sensor surface, in plaques-forming unit (pfu); that has allowed us to compare the number of phages determined by DLA and the estimated by the frequency shift, and both were very similar. With the aim to obtain a short response time, the detection of the phage was carried out in dynamic mode. The range tested concentrations has varied between 2x10^10 pfu*ml^-1 and 5x10^8 pfu*ml^-1. A fast response and a good discrimination among concentrations have been achieved. Besides, the AM13 has been used as secondary antibody in order to measure lower concentrations, such as 5*10^7 pfu*ml^-1.
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Submitted on : Sunday, February 17, 2013 - 5:21:27 PM
Last modification on : Thursday, August 16, 2018 - 11:44:02 AM

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D. Matatagui, M. Fernandez, J. Fontecha, J. Santos, M. Horrillo, et al.. Development of a Love-wave Immunosensor with Microfluidic Technology to Detect Phages in Dynamic Mode. 14th International Meeting on Chemical Sensors (IMCS 2012), May 2012, Nürnberg, Germany. pp.892-895, ⟨10.5162/IMCS2012/P1.2.3⟩. ⟨hal-00789252⟩

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