TNPO3 is required for HIV-1 replication after nuclear import but prior to integration and binds the HIV-1 core.

Jose Carlos Valle-Casuso (1) , Francesca Di Nunzio (2) , Yang Yang (1) , Natalia Reszka (1) , Maritza Lienlaf (1) , Nathalie Arhel (2) , Patricio Perez (1) , Abraham L Brass (3) , Felipe Diaz-Griffero (1)

1 Department of Microbiology and Immunology
2 Virologie moléculaire et vaccinologie
3 Ragon Institute of Massachusetts General Hospital

TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to the HIV-1 core. Overall, this work suggests that TNPO3 interacts with the incoming HIV-1 core in the cytoplasm to assist a process that is important for HIV-1 infection after nuclear import.

Domaines: Virologie

Jose Carlos Valle-Casuso, Francesca Di Nunzio, Yang Yang, Natalia Reszka, Maritza Lienlaf, et al.. TNPO3 is required for HIV-1 replication after nuclear import but prior to integration and binds the HIV-1 core.. Journal of Virology, 2012, 86 (10), pp.5931-6. ⟨10.1128/JVI.00451-12⟩. ⟨hal-00762028⟩