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Article Dans Une Revue Biophysical Journal Année : 2012

Differential Dynamic Microscopy: a High-Throughput Method for Characterizing the Motility of Microorganism

Résumé

We present a fast, high-throughput method for characterizing the motility of microorganisms in 3D based on standard imaging microscopy. Instead of tracking individual cells, we analyse the spatio-temporal fluctuations of the intensity in the sample from time-lapse images and obtain the intermediate scattering function (ISF) of the system. We demonstrate our method on two different types of microorganisms: bacteria, both smooth swimming (run only) and wild type (run and tumble) Escherichia coli, and the bi-flagellate alga Chlamydomonas reinhardtii. We validate the methodology using computer simulations and particle tracking. From the ISF, we are able to extract (i) for E. coli: the swimming speed distribution, the fraction of motile cells and the diffusivity, and (ii) for C. reinhardtii: the swimming speed distribution, the amplitude and frequency of the oscillatory dynamics. In both cases, the motility parameters are averaged over \approx 10^4 cells and obtained in a few minutes.

Dates et versions

hal-00749047 , version 1 (06-11-2012)

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Vincent A. Martinez, Rut Besseling, Ottavio A. Croze, Julien Tailleur, Mathias Reufer, et al.. Differential Dynamic Microscopy: a High-Throughput Method for Characterizing the Motility of Microorganism. Biophysical Journal, 2012, 103, pp.1637. ⟨10.1016/j.bpj.2012.08.045⟩. ⟨hal-00749047⟩
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