Towards the simplification of protein synthesis: iterative solid-supported ligations with concomitant purifications.

Abstract : Please release me: A new linker for the temporary tagging of peptides at their N-terminus after solid-phase elongation, and its potential for capture/release purification is demonstrated. This concept is extended to a remarkably efficient self-purifying N-to-C iterative triazole ligation strategy, which is applied to the synthesis of a polypeptide having 160 residues, in a high purity without the need for chromatographic purification (see picture; orange blocks: peptide segments).
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Journal articles
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https://hal.archives-ouvertes.fr/hal-00748170
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Submitted on : Monday, November 5, 2012 - 9:01:36 AM
Last modification on : Tuesday, June 25, 2019 - 3:36:13 PM

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Vincent Aucagne, Ibai E Valverde, Philippe Marceau, Mathieu Galibert, Nabil Dendane, et al.. Towards the simplification of protein synthesis: iterative solid-supported ligations with concomitant purifications.. Angewandte Chemie (English Edition), John Wiley & Sons, 2012, 51 (45), pp.11320-4. ⟨10.1002/anie.201206428⟩. ⟨hal-00748170⟩

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