Impact of RGD Nanopatterns Grafted onto Titanium on Osteoblastic Cell Adhesion

Abstract : This work reports on the synthesis of titanium bone implants functionalized with nanoparticles (NPs) containing Arg-Gly-Asp-Cys peptide (RGDC) and shows the adhesion behavior of cells seeded on these materials. RGDC peptides were first: conjugated to a norbornenyl-poly(ethylene oxide) macromonomer (Nb-PEO). Then, functional NPs with a size of similar to 300 nm and constituted of polynorbornene core surrounded by poly(ethylene oxide) shell were prepared by ring-opening metathesis polymerization in dispersed medium. The grafting density of these NPs on the titanium surface is up to 2 m(-2) (80 pmol of RGDC per cm(-2) of NP surface). Cell adhesion was evaluated using preosteoblast cells (MC3T3-E1). Results of cells cultured for 24 h showed that materials grafted with NPs functionalized with RGDC peptides enhance specific cell adhesion and can create filopodia-like among NP sites by stressing the cells.
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Submitted on : Monday, October 22, 2012 - 2:26:57 PM
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Minh Ngoc Nguyen, Thomas Lebarbé, Omar Farouk Zouani, Loïc Pichavant, Marie-Christine Durrieu, et al.. Impact of RGD Nanopatterns Grafted onto Titanium on Osteoblastic Cell Adhesion. Biomacromolecules, American Chemical Society, 2012, 13 (3), pp.896-904. ⟨10.1021/bm201812u⟩. ⟨hal-00744166⟩



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