Hybrid polymer/lipid vesicles: fine control of the lipid and polymer distribution in the binary membrane

Maud Chemin (1) , Pierre-Marie Brun (1) , Sébastien Lecommandoux (1, 2) , Olivier Sandre (1, 2) , Jean-François Le Meins (1, 2)

1 LCPO - Laboratoire de Chimie des Polymères Organiques
2 Team 3 LCPO : Polymer Self-Assembly & Life Sciences

Hybrid polymer/lipid giant unilamellar vesicles (GUVs) were developed using lipids of respectively low and high melting transition temperature (DPPC: 1,2-dipalmitoyl-sn-glycero-3 phosphocholine, T-m = 41 degrees C, and POPC: palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; T-m = -2 degrees C) and a copolymer poly(dimethylsiloxane)-graft-poly(ethylene oxide) (PDMS-g-PEO) well known to self-assemble into vesicular structures. Using epifluorescence microscopy as well as differential scanning calorimetry (DSC), different structures have been identified depending on the molar composition and on the fluid or gel state of the lipid used. The most promising objects are hybrid vesicles with copolymer as the major component, in which lipids are either randomly distributed or present "raft-like'' domains in the polymer-rich membrane. The results are discussed on the basis of the fluidity of the different components and of their respective membrane thicknesses

Mots clés: TRIBLOCK COPOLYMER VESICLES; BLOCK-COPOLYMERS; BIODEGRADABLE POLYMERSOMES; FLUID MEMBRANES; DRUG-DELIVERY; LIPOSOMES; ADHESION; PERMEABILITY; STABILITY; MICELLES

Domaines: Polymères

Maud Chemin, Pierre-Marie Brun, Sébastien Lecommandoux, Olivier Sandre, Jean-François Le Meins. Hybrid polymer/lipid vesicles: fine control of the lipid and polymer distribution in the binary membrane. Soft Matter, 2012, 8 (10), pp.2867-2874. ⟨10.1039/c2sm07188f⟩. ⟨hal-00744120⟩