Non-invasive long-term and real-time analysis of endocrine cells on micro-electrode arrays

Matthieu Raoux (1) , Yannick Bornat (2) , Adam Quotb (2) , Bogdan Catargi (3) , Sylvie Renaud (2) , Jochen Lang (1)

1 IECB - Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie
2 IMS - Laboratoire de l'intégration, du matériau au système
3 CHU Bordeaux

Non-invasive high-throughput and long-term monitoring of endocrine cells is important for drug research, phenotyping, tissue engineering and pre-transplantation quality control. Here we report a novel approach to obtain simultaneous long-term electrical recordings of different islet cell types using multi-electrode arrays. We implemented wavelet transforms to resolve the low signal/noise ratio inherent to these measurements and extracted on-line a signature specific of cell activity. The architecture employed allows multiplexing a large number of electrodes for high-throughput screening. This method should be of considerable advantage in endocrine research and may be extended to other excitable cells previously not accessible to the technique.

Domaines: Electronique

Matthieu Raoux, Yannick Bornat, Adam Quotb, Bogdan Catargi, Sylvie Renaud, et al.. Non-invasive long-term and real-time analysis of endocrine cells on micro-electrode arrays. The Journal of Physiology, 2011, 590 (5), pp.1085-1091. ⟨hal-00742984⟩